Table 1 Group 2 results. Two-way ANOVA followed by Bonferroni’s test for multiple comparisons comparing tumor volume, viscoelasticity |G*| and phase angle Y of tumors, NABT and the peripheral rim surrounding the tumor (“periphery”) between animals treated with B20 anti-VEGF antibody and untreated controls. NABT, normal appearing brain tissue; SD, standard deviation
Results two-way ANOVA | Results Bonferroni’s post-test | ||||||||
|---|---|---|---|---|---|---|---|---|---|
sum of squares | degree of freedom | mean squares | F | p-value | mean 1 (SD) | mean 2 (SD) | adjusted p-value | ||
tumor volume | |||||||||
interaction | 1883 | 2 | 941.4 | 64.79 | < 0.0001 | untreated | in mm3 | in mm3 | |
time | 3569 | 2 | 1785 | 122.8 | < 0.0001 | d6 vs. d8 | 25.6 (8.1) | 46.2 (10.2) | < 0.0001 |
treatment | 7614 | 1 | 7614 | 50.17 | 0.0001 | d6 vs. d10 | 25.6 (8.1) | 71.6 (11.4) | < 0.0001 |
d8 vs. d10 | 46.2 (10.2) | 71.6 (11.4) | < 0.0001 | ||||||
treated | |||||||||
d6 vs. d8 | 12.2 (4.4) | 16.0 (4.0) | 0.42 | ||||||
d6 vs. d10 | 12.2 (4.4) | 19.6 (5.0) | 0.02 | ||||||
d8 vs. d10 | 16.0 (4.0) | 19.6 (5.0) | 0.47 | ||||||
viscoelasticity |G*| tumor | |||||||||
interaction | 1.46 | 2 | 0.73 | 2.50 | 0.11 | untreated | in kPa | in kPa | |
time | 7.49 | 2 | 3.74 | 12.87 | 0.0014 | d6 vs. d8 | 6.8 (0.7) | 6.0 (0.6) | 0.6 |
treatment | 6.34 | 1 | 6.34 | 8.12 | 0.0215 | d6 vs. d10 | 6.8 (0.7) | 5.1 (0.7) | 0.0005 |
d8 vs. d10 | 6.0 (0.6) | 5.1 (0.7) | 0.04 | ||||||
treated | |||||||||
d6 vs. d8 | 7.1 (0.7) | 7.2 (0.3) | > 0.9 | ||||||
d6 vs. d10 | 7.1 (0.7) | 6.4 (0.9) | 0.2 | ||||||
d8 vs. d10 | 7.2 (0.3) | 6.4 (0.9) | 0.09 | ||||||
phase angle Y tumor | |||||||||
interaction | 0.01 | 2 | 0.01 | 3.16 | 0.07 | untreated | normalized | normalized | |
time | 0.01 | 2 | 0.01 | 7.18 | 0.0078 | d6 vs. d8 | 0.33 (0.02) | 0.30 (0.01) | 0.04 |
treatment | 0.01 | 1 | 0.01 | 12.84 | 0.0071 | d6 vs. d10 | 0.33 (0.02) | 0.29 (0.03) | 0.02 |
d8 vs. d10 | 0.30 (0.01) | 0.29 (0.03) | > 0.9 | ||||||
treated | |||||||||
d6 vs. d8 | 0.35 (0.02) | 0.36 (0.03) | > 0.9 | ||||||
d6 vs. d10 | 0.35 (0.02) | 0.33 (0.03) | 0.15 | ||||||
d8 vs. d10 | 0.36 (0.03) | 0.33 (0.03) | 0.04 | ||||||
viscoelasticity |G*| NABT | |||||||||
interaction | 1.73 | 2 | 0.87 | 8.62 | < 0.01 | untreated | in kPa | in kPa | |
time | 2.43 | 2 | 1.21 | 12.06 | < 0.01 | d6 vs. d8 | 7.7 (0.22) | 8.5 (0.37) | 0.0045 |
treatment | 1.33 | 1 | 1.33 | 3.36 | 0.99 | d6 vs. d10 | 7.7 (0.22) | 7.2 (0.34) | 0.08 |
d8 vs. d10 | 8.5 (0.37) | 7.2 (0.34) | < 0.0001 | ||||||
treated | |||||||||
d6 vs. d8 | 7.7 (0.56) | 7.9 (0.53) | 0.8 | ||||||
d6 vs. d10 | 7.7 (0.56) | 7.8 (0.56) | > 0.9 | ||||||
d8 vs. d10 | 7.9 (0.53) | 7.8 (0.56) | > 0.9 | ||||||
phase angle Y NABT | |||||||||
interaction | 0.01 | 2 | 0.01 | 3.07 | 0.07 | untreated | normalized | normalized | |
time | 0.01 | 2 | 0.01 | 0.27 | 0.76 | d6 vs. d8 | 0.40 (0.02) | 0.37 (0.04) | 0.16 |
treatment | 0.01 | 1 | 0.01 | 0.56 | 0.47 | d6 vs. d10 | 0.40 (0.02) | 0.37 (0.04) | 0.42 |
d8 vs. d10 | 0.37 (0.04) | 0.37 (0.04) | > 0.9 | ||||||
treated | |||||||||
d6 vs. d8 | 0.37 (0.02) | 0.39 (0.01) | 0.88 | ||||||
d6 vs. d10 | 0.37 (0.02) | 0.39 (0.02) | 0.64 | ||||||
d8 vs. d10 | 0.39 (0.01) | 0.39 (0.02) | > 0.9 | ||||||
viscoelasticity |G*| periphery | |||||||||
interaction | 0.48 | 2 | 0.24 | 0.55 | 0.59 | untreated (in kPa) | treated (in kPa) | ||
time | 3.6 | 2 | 1.8 | 4.16 | 0.04 | untreated vs. treated d6 | 7.1 (0.54) | 6.99 (0.31) | > 0.9 |
treatment | 0.17 | 1 | 0.17 | 0.22 | 0.65 | untreated vs. treated d8 | 7.3 (0.89) | 7.4 (0.59) | > 0.9 |
untreated vs. treated d10 | 6.3 (1.12) | 6.7 (0.69) | 0.9 | ||||||
phase angle Y periphery | |||||||||
interaction | 0.01 | 2 | 0.01 | 1.31 | 0.30 | untreated | treated | ||
time | 0.01 | 2 | 0.01 | 1.69 | 0.22 | untreated vs. treated d6 | 0.33 (0.01) | 0.34 (0.03) | > 0.9 |
treatment | 0.01 | 1 | 0.01 | 0.90 | 0.08 | untreated vs. treated d8 | 0.31 (0.02) | 0.34 (0.04) | 0.47 |
untreated vs. treated d10 | 0.32 (0.02) | 0.31 (0.01) | > 0.9 | ||||||