Fig. 4

3D appearance of an IPNM with finger-like protrusions. A representative virtual 2D X-ray based slice of the punch biopsy is shown (a). Semi-automated segmentation of the lumen of the papillary duct structure in a virtual 2D section (a, right green) as well as over the entire punch biopsy volume is presented in the form of an endocast. (b, green). The corresponding H&E stained section demonstrated a general overview of the different tissue structures, Movat’s pentachrome stain visualized connective tissue, mucines, and muscle cells and specific antibody staining with anti CK-18 antibody displayed all epithelial cells (c)