Fig. 1

Schematic workflow. First, the area of interest was identified from H&E stained 2 μm tissue slices obtained from the patient paraffin tissue block (a, blue circle). A 3.5 mm punch biopsy was taken from the selected area (b). The paraffin tissue cylinder was removed from the punch and directly mounted on a brass pin (c). A representative 2D slice through the volume of the PCCT scan of the biopsy is shown, recorded at the P10/PETRA III synchrotron source (DESY, Hamburg, Germany). To this end, 9 individual tomograms with overlapping regions were stitched together subsequently (d, blue grid). Afterwards the punch biopsies were again embedded in paraffin (e). For correlative analysis, 2 μm paraffin sections were sliced and stained with H&E (f)