Fig. 2

Method of calculating ultrasonic RF time series parameters. a An RF time series is formed by temporal ultrasonic signals that are collected continuously from a steadfast ROI (the red rectangle) of tumor tissue. b The parameters S1, S2, S3 and S4 were the integral of the normalized average spectrum of the ROI in each quarter of the normalized frequency range (divided by the blue line). The parameters slope and intercept were the slope and intercept of the regression line (the green line) fitted to values of the spectrum